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Enhance Your Publication with Imaris Snapshot

The Imaris 'Snapshot' feature captures a 2D image of your 2D, 3D or 4D image data as it currently appears in the viewing area. It creates a new file containing your image, all scale information and measurement data that are displayed.

Knowing all of Snapshot's features can enhance your work by creating the optimal published document.

You can access the Snapshot view either by choosing the Snapshot option from the File menu or simply by clicking on the Snapshot icon

The Snapshot preview offers a view of the snapshot content, as it will look when finalized. The Image Size settings allow you to choose and adjust the content and the size of the snapshot. The default size setting is the Snapshot size from window size option. When this option is selected, the snapshot dimensions match the width/height of the viewing area. Resizing the view area alters the snapshot dimensions accordingly.

To modify and customize the snapshot dimensions you can either select the predefined sets from the drop-down list or manually adjust the Width and Height values. Adjusting the width or height settings for the snapshot changes the number of pixels in the snapshot and scales the input image. The scaled image is centered within the new snapshot size and the zoom of the input image is automatically adjusted to better fit the available pixels in the snapshot. By changing the snapshot size the image in the viewing area is either re-sampled cropped and/or expanded to match the specified pixel dimensions.

Depending on the settings, a difference between the dimensions of the viewing area and the snapshot size could occur. In these situations, the snapshot is surrounded with bars in the color of the Imaris screen background.

There are two options to adjust the content of the snapshot and to eliminate the screen background. The image can be either scaled to fit the selected snapshot dimensions (by adjusting the zoom in the viewing area) or else the Crop to fill whole snapshot area option could be selected.

The Crop to fill whole snapshot area option crops the image to fit the snapshot specified Width and Height values. It sets an image in a way that one dimension fits the snapshots viewing area, while the overflow of the other dimension is cut off, respecting image aspect ratio. The images below illustrate Pyramidal cell image snapshots taken with the various settings; inconsistency between image size and snapshot intended dimensions, the same snapshot size adjusted with the Crop to fill whole snapshot area option.

The 100% Snapshot button provides an easy way to standardize snapshot scaling, giving you the opportunity to handle and compare different effects easily by superimposing multiple snapshots. Selecting this button ensure that multiple snapshots always have the same scale factor, resulting in snapshots with the zoom factor set to 1 (pixel per voxel).

When the snapshot is loaded into an external application to prepare the image for printing the Resolution (dpi) option lets you adjust the rendering quantity of the snapshot. For example, if the snapshot size is 512 x 512 pixels, and the resolution value is set to 100 dpi, then the default print size will be 5.12 inches x 5.12 inches.

To create the snapshot, the Image Output format should also be selected. Imaris provide the multiple output options: Save to File, Copy to Clipboard or Add to ImageAccess Database.

When the option Save to File is selected, clicking on the Do Snapshot! button saves the snapshot to the file and folder specified in the snapshot name field. You can either accept the automatic naming suggestion or enter a new name. When the Copy to Clipboard is selected, clicking on the Do Snapshot! button copies the snapshot to the clipboard, while selecting the option 'Add to ImageAccess Database' provides the option to manage the snapshots more efficiently by adding them to the database.

Date: April 2011

Category: How-to Guides

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