Imaris ClearView-GPU™ Deconvolution

Did you know that images coming from fluorescent microscopes are a convolution of the real (original) image and point spread function (PSF)? See the sharp edges of your structures and remove the haze around your objects by restoring the original image using the mathematical operation called deconvolution. Deconvolve your data and get a sharper image using Imaris ClearView-GPU™.

Put Those Photons Back Where They Came From!

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ClearView-GPU™ Microscopy Deconvolution Benefits

ClearView-GPU™ is intuitive and easy, makes use of the existing hardware due to GPU acceleration and can be run in batch on multiple datasets.

ClearView-GPU™ is fast – GPU accelerated (watch video)
ClearView-GPU™ is anchored in the Imaris analysis workflow (watch video)
ClearView-GPU™ is batchable (watch video)
ClearView-GPU™ is automatic - minimal user input required (watch video)

Deconvolution for Widefield, Confocal and TIRF Microscopy Imaging

ClearView-GPU™ deconvolution has dedicated algorithms for: confocal, widefield and TIRF and can be performed on 3D stacks and time series.

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Point Scanning Confocal - Image courtesy of: Dr Claudia Florindo and Dr Álvaro Tavares, CMBR, University of Algarve, Algarve, Portugal

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Spinning Disk Confocal - Image courtesy of Edinburgh Super Resolution Imaging Facility (ESRIC), Edinburgh, UK

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Spinning Disk Confocal - Image courtesy of University of Montpellier, Montpellier, France

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Spinning Disk Confocal - Image courtesy of: Dr Shin-ichi Koizumi, Okinawa Institute of Science and Technology (OIST), Okinawa, Japan

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Widefield - Image courtesy of: Dr Mark Brown, Andor Technology, Belfast, UK

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Widefield, Image courtesy of Biomedical Imaging Group, EPFL, Lausanne, Switzerland

ClearView-GPU™ Deconvolution Technical Features and Algorithms

Versatile - Optimized PSF for widefield, point scanning confocal and spinning disk confocal microscopy,
Powerful – GPU-accelerated enhanced Richardson-Lucy and Inverse filtering algorithms,
Accurate - Accelerated Gibson-Lanni algorithm for accurate PSF estimates, supporting spherical aberration correction in deep specimens,
Extremely fast - Optimized CUDA workflow for scorching performance,
Quantitative - “Energy conservation” matches total photon content of raw data and results,
Innovative - Richardson-Lucy iteration acceleration with gradient-driven convergence in fewer cycles.

Read Technical Article Watch Tutorial

Integrated into Dragonfly Fusion

ClearView-GPU™ deconvolution module is part of the Fusion acquisition software which runs the Andor Dragonfly multi-modal confocal, which enables performing almost live deconvolution during acquisition. That said, Dragonfly not only benefits from Andor EMCCD and sCMOS technologies and spinning disk to deliver industry-leading signal to noise and image fidelity but also ClearView-GPU™ deconvolution to maximize resolution and throughput. ClearView-GPU™ deconvolution can be applied in all modes that Dragonfly operates: confocal, widefield and TIRF on 3D stacks and time series.

ClearView-GPU™ module is part of the Fusion acquisition software which runs the Andor Dragonfly multi-modal confocal. ClearView-GPU™ enables immediate live deconvolution during acquisition and visualisation in real-time during the experimental progress.

Clearview-GPU™ Deconvolution Learning Resources

Keen to learn more? Browse a selection of learning material below.

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