Imaris 8.2 Release Notes

Welcome to the Imaris 8.2 release notes. Please have a look to the overview of Imaris Release notes for information about prior released features and fixed bugs.

Version Date: December 16, 2015

Lineage Tracking Algorithm

Imaris8.2 has a lineage tracking algorithm that allows to track dividing cells. The lineage tracking algorithm is available on the tracking tab of Spots, Surfaces, Cells or Filaments.

The screenshot below shows spots tracked with the lineage tracking algorithm and colored by “TrackID”. Spots with the same color descend from the same mother cell.

Track Editor with Lineage Tree Visualization and Statistics Display

To complement the lineage tracking functionality Imaris8.2 has a new track editor that displays tracks as “lineage tree” in such a way that the number of intersections in the display is minimal (often zero).

The track editor provides useful functionally to manually edit tracks using "connect" and "disconnect" and to arrange the lineage tree in different ways by moving tracks left or right or flipping the orientation of branches. The new track editor has the functionality to overlay colors on the tracks. This can be used to display any statistical measurement value as a color on the tracks. It enables for example a display of the intensity of a second channel for each tracked object.

The “Object Labeling” feature described in the next section can be used to highlight certain parts of a lineage tree as shown in this figure:

Object Labeling for Coloring and Statistics Export

Users can add labels to 3D objects such as spots, surfaces, cells, vesicles, etc. The labels can be used to color code objects. They are also added to the statistics of each object as a factor that appears in the statistics tables and export. In the screenshot below the blue objects have been labeled as “Fast” and the orange objects have been labeled as “Slow”. The colors are used for rendering in the 3D view. The label names are displayed in the statistics.

FileReaders

• New OIR file reader
• Slidebook updated to format version 6
• PrairieView updated to format version 5.2
• New Fiji Big Data Viewer reader
• Updated OME Tiff reader

Fast FileSave with Parallel gzip Compression

Saving files in version 8.2 is significantly faster (up to a factor of 5) than previous versions due to multithreaded gzip compression of image data. This applies to both Imaris and ImarisFileConverter.

Time Slider Displays Frame Number

ObjectIDs remain unchanged during edit and delete

The objectIDs that Imaris assigns to spots, surfaces, cells, nuclei and vesicles do not change when an object is deleted or added or the whole container is copied. As a consequence of this the IDs no longer necessarily span an entire range of numbers. Suppose you have 4 spots with IDs 1,2,3,4 and you delete the one with ID 2, then the remaining IDs are 1,3,4.

XTInterface

There is a new function “GetIds” which returns the ObjectIDs used in statistics.

Fixed Bugs